中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (27): 5127-5130.doi: 10.3969/j.issn.1673-8225. 2010.27.044

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    

牡蛎提取物在高温诱导皮质神经干细胞凋亡中的保护作用

宋海岩1，宋勇莉2，于清梅3，庄 园3，武玉玲4   

  1. 1新乡医学院人体解剖学教研室，河南省新乡市  453003；2山东中医药大学第二附属医院预防保健科，山东省济南市  250001；3山东医学专科学校解剖教研室，山东省临沂市  276002；4山东大学医学院组织与胚胎学教研室，山东省济南市  250012
  • 出版日期:2010-07-02 发布日期:2010-07-02
  • 通讯作者: 武玉玲，教授，硕士生导师，山东大学医学院组织与胚胎学教研室，山东省济南市 250012 wylp53@sdu.edu.cn
  • 作者简介:宋海岩★，女，1980年生，河南省卫辉市人，汉族，2007年山东大学毕业，硕士，助教，主要从事神经解剖学的研究。 zzx@xxmu.edu.cn
  • 基金资助:

    山东省科技厅项目(2005GG4402054)。

Protective effect of oyster extract on apoptosis of cerebral neural stem cells induced by hyperthermia

Song Hai-yan1, Song Yong-li2, Yu Qing-mei3, Zhuang Yuan3, Wu Yu-ling4   

  1. 1 Department of Human Anatomy, Xinxiang Medical University, Xinxiang  453003, Henan Province, China; 2 Department of Prevention and Health Care, Second Affiliated Hospital of Shandong Chinese Medical University, Jinan  250001, Shandong Province, China; 3 Department of Human Anatomy, Shandong Medical College, Linyi  276002, Shandong Province, China; 4 Department of Histology and Embryology, Shandong University Medical School, Jinan  250012, Shandong Province, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Wu Yu-ling, Professor, Master’s supervisor Department of Histology and Embryology, Shandong University Medical School, Jinan 250012, Shandong Province, China wylp53@sdu.edu.cn
  • About author: Song Hai-yan★, Master, Assistant, Department of Human Anatomy, Xinxiang Medical University, Xinxiang 453003, Henan Province, China zzx@xxmu.edu.cn
  • Supported by:

    the Science and Technology Department of Shandong Province, No. 2005GG4402054*

PDF

339

被引次数

3

摘要：

背景: 课题组前期研究表明，牡蛎提取物对高温所致神经管畸形中的凋亡细胞有一定的保护作用，该提取物对体外培养的神经干细胞的凋亡是否也有保护作用，尚未见报道。
目的：观察牡蛎提取液在高温所致神经干细胞凋亡中的保护作用。
方法：取孕13 d小鼠胚胎大脑皮质细胞培养，采用免疫荧光法检测巢蛋白的表达以鉴定神经干细胞，将培养3代的神经干细胞随机分为4组：高温对照组和牡蛎保护Ⅰ、Ⅱ、Ⅲ组(分别加入质量浓度2.5，5，10 g/L的牡蛎提取液)，同时设立培养液对照组(其中无细胞)、培养液+牡蛎提取液对照组(其中无细胞)，各牡蛎保护组及对照组均经过39 ℃高温处理。锥虫蓝染色法检测神经干细胞存活率；MTT法检测神经干细胞的增殖活性；蛋白质印迹方法检测神经干细胞凋亡相关蛋白p53的表达。
结果与结论：牡蛎保护Ⅱ、Ⅲ组中细胞的存活率及MTT值明显高于高温对照组(P < 0.05)；而p53的表达则明显低于高温对照组。提示牡蛎提取液对高温所致神经干细胞的凋亡具有一定的保护作用，这种作用有浓度依赖性。

关键词: 牡蛎, 神经干细胞, 高温, 小鼠, 凋亡

Abstract:

BACKGROUND: Previous results of our study show that oyster extract has some protective effects on apoptosis of the neuroepithelium in neural tube defects induced by hyperthermia in vivo. It remains unclear whether the extract also protects in vitro cultured neural stem cells.
OBJECTIVE: To investigate the protective effect of oyster extract on apoptosis of cerebral neural stem cells induced by hyperthermia.
METHODS: The cerebral neural stem cells of embryonic mice of 13 days were cultured in vitro. Nestin expression was detected by immunofluorescence method to identify neural stem cells. The neural stem cells of passage 3 were divided randomly into 4 groups: hyperthermia control group and oyster treated I, II, III groups (mass concentration 2.5, 5, 10 g/L oyster extract solution). In addition, culture solution control group (no cells), and culture solution+ oyster extract control group (no cells) were designed. All oyster extract groups and control groups were treated by hyperthermia over 39 °C. The survival rate and the vitality of neural stem cells were detected by trypan blue staining and MTT assay. Western-blotting was employed to explore the expression of p53 in cerebral neural stem cells of each group.
RESULTS AND CONCLUSION: The survival rate and the value of MTT assay in oyster treated groups II and III were significantly greater than hyperthermia control group (P < 0.05), but the expression of p53 in oyster treated groups II and III were weaker than hyperthermia control group. Oyster extract plays an important protective role in the apoptosis of neural stem cells induced by hyperthermia.

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